TatAD

• Description: component of the TatAD-TatCD twin-arginine translocase
  
  

Gene name	tatAD
Synonyms	yczB
Essential	no
Product	component of the twin-arginine translocation pathway
Function	TAT protein secretion
Gene expression levels in SubtiExpress: tatAD
Interactions involving this protein in SubtInteract: TatAD
Metabolic function and regulation of this protein in SubtiPathways: TatAD
MW, pI	7 kDa, 9.693
Gene length, protein length	210 bp, 70 aa
Immediate neighbours	phoD, tatCD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism, protein secretion, membrane proteins

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU02630

Database entries

• BsubCyc: BSU02630

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: translocation of PhoD PubMed

• Protein family: tatA/E family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • TatAD-PhoD PubMed
  • TatAD-TatCD, TatAD-TatCY PubMed
  • CsbC-TatAD PubMed
  • TatAC-TatAD PubMed
  • TatAY-TatAY PubMed

• Localization: membrane (according to Swiss-Prot), forms foci at the division sites and cell poles, localization requires interaction with TatCD or TatCY PubMed

Database entries

• BsubCyc: BSU02630

• Structure: 2L16 PubMed

• UniProt: O31467

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: phoD-tatAD-tatCD PubMed

• Expression browser: tatAD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:
  • expression of the operon depends on functional HemAT and CsbC PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

References

Reviews

Kenneth Cline
Mechanistic Aspects of Folded Protein Transport by the Twin Arginine Translocase (Tat).
J Biol Chem: 2015, 290(27);16530-8
[PubMed:25975269] [WorldCat.org] [DOI] (I p)

Vivianne J Goosens, Carmine G Monteferrante, Jan Maarten van Dijl
The Tat system of Gram-positive bacteria.
Biochim Biophys Acta: 2014, 1843(8);1698-706
[PubMed:24140208] [WorldCat.org] [DOI] (P p)

Tracy Palmer, Ben C Berks
The twin-arginine translocation (Tat) protein export pathway.
Nat Rev Microbiol: 2012, 10(7);483-96
[PubMed:22683878] [WorldCat.org] [DOI] (I e)