PHT01

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The pHT01 is a high-level intracellular expression vector for recombinant protein production with B. subtilis. The vector is based on the strong σ^A-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an E. coli/ B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

Features

• Efficiently controllable gene expression (IPTG inducible)
• High level production of recombinant proteins
• E. coli / B. subtilis shuttle vector

pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag) are available from MoBiTec GmbH (Göttingen)