smc
168
part of the [wiki|condensin] complex, chromosomal origin condensation and segregation SMC protein
locus
BSU_15940
Molecular weight
135.29 kDa
pI
5.27
function
segregation of replication origins
product
chromosome condensation and segregation SMC protein
essential
yes
synonyms
smc, ylqA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1196 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,666,560 1,670,120
Phenotypes of a mutant
essential [Pubmed|12682299]
terminal phenotype of a CRISPRi knockdown mutant is moderate lysis [Pubmed|27238023]
''[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]
''[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]'' lethality on complex medim can be suppressed by inactivation of ''[gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]'' and ''[gene|EA155B3BEB9EAC97114058E23B6D8CDF8823A602|tsaC]'' (constitutive [wiki|stringent response]) [Pubmed|26539825]
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The protein
Catalyzed reaction/ biological activity
ATPase activity, cycles of ATP hydrolysis are used by the [wiki|condensin] complex to extrude DNA loops [pubmed|30100265]
Protein family
SMC family (with [protein|ED825D5B3290BA8F9C92477B1FCC433ADB3C8449|sbcC], according to UniProt)
[wiki|Domains]
SMC hinge domain (aa 519-637) (according to UniProt)
Structure
[PDB|3ZGX] (complex between the head domain of [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]) [Pubmed|23353789]
[AF|P51834]
Effectors of protein activity
ATP-induced DNA-binding results in a more open conformation of the [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc] dimer, and thus of the [wiki|condensin] complex [Pubmed|25557547]
[wiki|Localization]
Nucleoid (heterogeneous) [pubmed|16479537]
The [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [pubmed|28154080],[pubmed|24440393],[pubmed|23475963]
Expression and Regulation
Operons
genes
[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]-[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-[gene|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY]
description
[Pubmed|11021934]
regulation
expressed during vegetative growth and during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|11021934]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11021934], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B148 (smc::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1147 NBRP B. subtilis, Japan]
1A822 ( ''smc''::pMUTIN ''trpC2'' Em), [Pubmed|12682299], available at [http://bgsc.org/getdetail.php?bgscid=1A822 the Bacillus Genetic Stock Center]
BEC15940 (''smc''::''erm trpC2''), a CRISPRi knockdown mutant, [Pubmed|27238023], is available at the [http://bgsc.org Bacillus Genetic Stock Center]
References
Reviews
Original Publications
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Time of last update: 2024-11-10 15:36:05
Author of last update: Jstuelk