sirA

sirA
168

[category|SW.4.2|Sporulation] protein, inhibits [category|SW.3.1.1|DNA replication] initiation in cells committed to [category|SW.4.2|Sporulation], facilitates capture of oriC in the forespore

locus
BSU_17900
Molecular weight
18.00 kDa
pI
7.35
Protein length
148 aaSequenceBlast
Gene length
447 bpSequenceBlast
function
control of chromosome copy number
product
inhibitor of [category|SW.3.1.1|DNA replication]
essential
no
synonyms
sirA, yneE, yoxF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
1,922,017  1,922,463
Phenotypes of a mutant
over-replication during [category|SW.4.2|Sporulation] [Pubmed|19682252]
defective in oriC segregation during [category|SW.4.2|Sporulation] [pubmed|27489185]
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The protein
Catalyzed reaction/ biological activity
interacts with domain I of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] and displaces it from the replication origin, resulting in inhibition of replication [Pubmed|19682252]
interacts with domain III of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] to facilitate [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA]-dependent oriC capture in the forespore [pubmed|27489185]
interaction with [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] prevents the [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-[protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD] interaction [pubmed|36416272]
Structure
[PDB|4TPS] (in complex with N-terminal domain of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]) [Pubmed|25041308]
[AF|P45707]
[wiki|Localization]
accumulates at the [wiki|replisome] in sporulating cells (perhaps in [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-dependent manner) [Pubmed|25041308]
Expression and Regulation
Operons
genes
[gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]-[gene|9C151A07F22920F58D635B82545DA76C7087AA8E|yneF]
description
[Pubmed|9068642]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647,19329632]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647,19329632], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
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[gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]→[gene|9C151A07F22920F58D635B82545DA76C7087AA8E|yneF]

2024-11-10 06:39:35

ghost

65

7be25bbf8b2860812c03b636554f14845ca3635d

07CD762FF4055D52724ECCC1516AFB4F25695D64

Biological materials
Mutant
MGNA-B389 (yneE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1388 NBRP B. subtilis, Japan]
BKE17900 ([gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC,  downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA
BKK17900 ([gene|03595BF6DB8AE79604A720A5BD85C0DB781EE306|sirA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTCATCCCCTTTTC,  downstream forward: _UP4_TAAAACCGAAGAAAAAGAGA
References
Reviews
20157337,22933559,28075389
Original Publications
19682252,21239581,14651647,19329632,9068642,25041308,27489185,30285297,33510358,36416272,38548820

03595BF6DB8AE79604A720A5BD85C0DB781EE306

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Time of last update: 2024-09-18 16:56:33

Author of last update: Jstuelk