panE
168
ketopantoate reductase
locus
BSU_15110
Molecular weight
33.13 kDa
pI
5.86
function
biosynthesis of coenzyme A
product
ketopantoate reductase
essential
no
ec
1.1.1.169
synonyms
ylbQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1893 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,577,409 1,578,305
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
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The protein
Catalyzed reaction/ biological activity
2-dehydropantoate + H+ + NADPH --> (R)-pantoate + NADP+ [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]
Protein family
ketopantoate reductase family (with [protein|60264E4BE76F5995A8317294D05FC27F8B34E09D|panG], according to UniProt)
Structure
[PDB|3EGO]
[AF|O34661]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]-[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]
description
[Pubmed|20308541]
regulation
''[protein|search|ylbQ]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B254 (ylbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1253 NBRP B. subtilis, Japan]
BKE15110 ([gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTCACACCCAATTT, downstream forward: _UP4_TGAGCTTTTTCGGTAACATG
BKK15110 ([gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATGTCACACCCAATTT, downstream forward: _UP4_TGAGCTTTTTCGGTAACATG
GP3384 (Δ[gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]::neo trpC2), available in [wiki|Jörg Stülke]'s lab
References
Operon and expression
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Time of last update: 2024-11-29 20:45:04
Author of last update: Jstuelk