panU
168
S protein of biotin [wiki|ECF transporter]
locus
BSU_10370
Molecular weight
19.87 kDa
pI
10.68
function
uptake of biotin
product
S protein of biotin [wiki|ECF transporter]
essential
no
synonyms
yhfU, bioY
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1268 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,111,925 1,112,485
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The protein
Catalyzed reaction/ biological activity
uptake of pantothenate (R)-pantothenate + ATP --> (R)-4'-phosphopantothenate + ADP + H+ [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]
Protein family
bioY family (with [protein|E98078C3CB9F19949009A8E50340E4C9E67663E6|yuiG], according to UniProt)
Structure
[PDB|4DVE] (BioY from ''Lactococcus lactis'', 35% identity, 71% similarity) [Pubmed|22891302]
[AF|O07620]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|22215066A7BEA4E53189DE6639C19D1AD86DA7F4|panU]-[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
description
[Pubmed|22383849]
regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|11717296]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|11717296], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
additional information
weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
Biological materials
Mutant
MGNA-B281 (yhfU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1280 NBRP B. subtilis, Japan]
BKE10370 ([gene|22215066A7BEA4E53189DE6639C19D1AD86DA7F4|panU]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAATCCTCCAAAAA, downstream forward: _UP4_TTTACAAAAGGAGGATGACA
BKK10370 ([gene|22215066A7BEA4E53189DE6639C19D1AD86DA7F4|panU]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAATCCTCCAAAAA, downstream forward: _UP4_TTTACAAAAGGAGGATGACA
References
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Time of last update: 2024-11-30 14:23:31
Author of last update: Jstuelk