spoVS
168
required for dehydratation of the spore core and assembly of the coat, mutation increases [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-dependent gene expression, might act via [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]
locus
BSU_16980
Molecular weight
8.66 kDa
pI
6.47
function
spore coat assembly, spore core dehydratation
product
unknown
essential
no
synonyms
spoVS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2359 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,769,935 1,770,195
Phenotypes of a mutant
increased [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-dependent gene expression, this might occur via [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] [Pubmed|16428420]
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The protein
Structure
[PDB|2EK0] (from ''Thermus thermophilus'', 56% identity)
[AF|P45693]
Expression and Regulation
Operons
genes
[gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]
description
[Pubmed|7559352]
regulation
expressed at the onset of stationary phase ([protein|search|SigH]) [Pubmed|7559352]
sigma factors
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|7559352], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Biological materials
Mutant
GP1601 ([gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::cat), available in [wiki|Jörg Stülke]'s lab
GP1848 ([gene|154862E3FFA7CEBA5725BAD08E09D0E1C6B7F946|ymdB]-[gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::''spc'' cassette), available in [wiki|Jörg Stülke]'s lab
BKE16980 ([gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTGCTCCCCCTTAGT, downstream forward: _UP4_TAAAAACAAATAAAGCATTC
BKK16980 ([gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTGCTCCCCCTTAGT, downstream forward: _UP4_TAAAAACAAATAAAGCATTC
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-07 16:31:45
Author of last update: Jstuelk