uvrA
168
excinuclease ABC (subunit A), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations
locus
BSU_35160
Molecular weight
105.83 kDa
pI
5.84
function
DNA repair after UV damage
product
excinuclease ABC (subunit A)
essential
no
synonyms
uvrA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0178 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,610,064 3,612,937
Phenotypes of a mutant
reduced stationary phase mutagenesis [Pubmed|27399782]
a [gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA] [gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB] mutant is sensitive to blue light-induced DNA damage [pubmed|30054368]
sensitive to Cr(VI) treatment [pubmed|30745368]
reduced resistance towards electron beams [pubmed|31948638]
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The protein
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|Domains]
2 [wiki|ABC transporter domain]s (aa 309-587, aa 607-935) (according to UniProt)
Structure
[PDB|2R6F] (Geobacillus stearothermophilus)
[AF|O34863]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB]-[gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]
description
[Pubmed|22383849]
regulation
induced upon DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|8226626]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|8226626], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
additional information
during [protein|search|sporulation] expressed both in the forespore and the mother cell [PubMed|24118570]
expression is induced in the presence of Cr(VI) [pubmed|30745368]
Biological materials
Mutant
GP3534 (Δ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]-[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB])::kan), available in [wiki|Jörg Stülke]'s lab
GP1175 (Δ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]-[gene|DEFBDC2AB8E43FB06C65389160E8EACFE5FB9705|uvrB])::ermC), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
BKE35160 ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCCGATCCATAGCCATTT, downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG
BKK35160 ([gene|39B73AADBFD42203ADFA2E834697799E6430A086|uvrA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCCGATCCATAGCCATTT, downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG
References
Reviews
Original Publications
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Time of last update: 2024-11-29 20:45:20
Author of last update: Jstuelk