nfo
168
type IV apurinic/apyrimidinic endonuclease
locus
BSU_25130
Molecular weight
32.92 kDa
pI
5.37
function
repair of oxidative DNA damage in spores
product
type IV apurinic/apyrimidinic endonuclease
essential
no
synonyms
nfo, yqfS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0648 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,593,281 2,594,174
Phenotypes of a mutant
an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]'' [Pubmed|24244006]
an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|fenA] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is sensitive to radiation [Pubmed|24123749]
sensitive to blue light-induced DNA damage [pubmed|30054368]
reduced resistance towards electron beams [pubmed|31948638]
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The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to UniProt)
binds to 8-oxoG, abasic, and RNA base DNA lesions [pubmed|37250769]
Protein family
AP endonuclease 2 family (single member, according to UniProt)
Structure
[PDB|3AAL] (from Geobacillus kaustophilus, 80% identity) [pubmed|21358045]
[AF|P54476]
Expression and Regulation
Operons
genes
[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]
description
[pubmed|22383849]
Biological materials
Mutant
GP899 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::''kan'') and GP1502 ([gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
GP1503 (Δ[gene|38CC84343C47CBF37F007E9056A9248869C396E1|exoA]::''kan'' [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
MGNA-C462 (yqfS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2460 NBRP B. subtilis, Japan]
available in [wiki|Mario Pedraza-Reyes]'s lab
BKE25130 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT, downstream forward: _UP4_TAAAAAATGGGGGATAACAC
BKK25130 (Δ[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT, downstream forward: _UP4_TAAAAAATGGGGGATAACAC
References
Reviews
Original Publications
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Time of last update: 2024-10-10 09:39:13
Author of last update: Jstuelk