shfA
168
sporulation heterogeneity factor Antidote, required for sporulation at a late stage
locus
BSU_00610
Molecular weight
24.95 kDa
pI
10.74
function
control of sporulation entry in neighbor cells
product
sporulation heterogeneity factor Antidote
essential
no
synonyms
yabQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5869 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
68,515 69,150
Phenotypes of a mutant
inactivation of [gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA] reduces sporulation efficiency to 0.1% that of wild type cells [Pubmed|26735940], this can be suppressed by inactivation of [gene|BA2FA842DAB19E32F48380F56BB772D7E60F2D28|shfP] [https://www.biorxiv.org/content/10.1101/2024.03.27.587046v1.full bioRxiv]
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The protein
Structure
[AF|P37559]
[wiki|Localization]
forespore outer membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|D6B1837075ECBEE1FEAC704DDB6795336092897F|yabM]-[gene|52D48F22CB53421EE30970D34B4861E2F8A5893D|yabN]-[gene|B0CB19A3E3E84E12BF42BB050394C8A059A89359|rqcP]-[gene|035B5486A3887FC71ACC0A8FB43ED77DC44E12EA|yabP]-[gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]-[gene|FE0E60B875E72AEF5174A89C7AE5556FF59B19D5|divIC]-[gene|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR]
description
[Pubmed|11283287]
regulation
expressed during sporulation ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|11283287]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,11283287], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|12207695], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|12207695], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
genes
[gene|035B5486A3887FC71ACC0A8FB43ED77DC44E12EA|yabP]-[gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]-[gene|FE0E60B875E72AEF5174A89C7AE5556FF59B19D5|divIC]-[gene|8EDB1BCEEE64FDAF1C9054300751FCB420658865|yabR]
description
[Pubmed|11283287]
regulation
expressed during sporulation ([protein|search|SigE]) [Pubmed|11283287]
additional information
there are about 50,000 molecules of DivIC per cell [http://www.ncbi.nlm.nih.gov/sites/entrez/9426141 PubMed]
Biological materials
Mutant
MGNA-B918 (yabQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1917 NBRP B. subtilis, Japan]
BKE00610 ([gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTATAGAATTGTGTCGTCA, downstream forward: _UP4_AGATGAAAGGAGGACCGTCT
BKK00610 ([gene|4DEDB27C0162409571AB4A1BB17CFE400764AE45|shfA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTATAGAATTGTGTCGTCA, downstream forward: _UP4_AGATGAAAGGAGGACCGTCT
References
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Time of last update: 2024-05-23 02:41:35
Author of last update: Jstuelk