rppH
168
decapping of NAD-capped RNAs, part of the error prevention oxidized guanine system (with [protein|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM] and [protein|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY]), confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity,
locus
BSU_30630
Molecular weight
18.37 kDa
pI
5.95
function
DNA repair, RNA degradation
product
8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase
essential
no
synonyms
rppH, mutTA, ytkD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1051 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,134,983 3,135,459
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The protein
Catalyzed reaction/ biological activity
removal of pyrophosphate from the 5' end of mRNAs to make RNA accessible for degradation by [wiki|RNases] [Pubmed|21925382]
RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position [Pubmed|23610425,23610407]
decapping of NAD-capped RNAs [pubmed|30110644]
8-oxo-dGTP + H2O --> 8-oxo-dGMP + diphosphate + H+ (according to UniProt)
Protein family
[wiki|Nudix hydrolase] (according to UniProt)
[wiki|Domains]
[wiki|Nudix hydrolase domain] (aa 6-145) (according to UniProt)
Structure
[PDB|4JZV] [Pubmed|23610407]
[AF|O35013]
Expression and Regulation
Operons
genes
[gene|50A50E33796EEF8046142EE928753C355EF42A1A|rppH]
description
[Pubmed|9387221]
regulation
expressed during logarithmic growth ([protein|search|SigA]) and early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|14761999], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|14761999], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
MGNA-A297 (ytkD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/297 NBRP B. subtilis, Japan]
BKE30630 ([gene|50A50E33796EEF8046142EE928753C355EF42A1A|rppH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG
BKK30630 ([gene|50A50E33796EEF8046142EE928753C355EF42A1A|rppH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG
References
Reviews
Original Publications
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Time of last update: 2024-11-29 20:45:27
Author of last update: Jstuelk