purH
168
phosphoribosylaminoimidazole carboxamide formyltransferase
locus
BSU_06520
Molecular weight
55.57 kDa
pI
5.13
function
purine biosynthesis
product
phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase
essential
no
ec
2.1.2.3
synonyms
purH, purJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0138 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
708,594 710,132
Phenotypes of a mutant
more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of [gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB] (reason: accumulation of ZTP that stimulates the binding of [protein|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA] to [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE], and thus folate biosynthesis during zinc limitation) [pubmed|31132310]
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The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide --> (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
H2O + IMP --> 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
Protein family
purH family (single member, according to UniProt)
[wiki|Domains]
MGS-like domain (aa 1-146) (according to UniProt)
Structure
[PDB|1ZCZ] (from ''Thermotoga maritima'', 36% identity, 54% similarity) [Pubmed|18260100]
[AF|P12048]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]-[gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]-[gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]-[gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]-[gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]-[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]-[gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]-[gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]-[gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]-[gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]-[gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]-[gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]
description
[Pubmed|3036807]
regulation
expression activated by glucose (4.4 fold) [Pubmed|12850135]
regulatory mechanism
G-box: RNA switch, ([wiki|riboswitch]) [pubmed|3036807,12787499], in [regulon|other_regulator:G-box|G-box]
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|7638212,2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3036807], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
BKK06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
References
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Time of last update: 2024-04-24 03:28:34
Author of last update: Melvin.boenninger