lutB
168
lactate catabolic enzyme
locus
BSU_34040
Molecular weight
53.31 kDa
pI
5.85
function
lactate utilization
product
lactate oxidase
essential
no
synonyms
lutB, yvfW
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1139 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,493,519 3,494,958
Phenotypes of a mutant
no growth with lactate as the single carbon source [Pubmed|19201793]
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The protein
Catalyzed reaction/ biological activity
oxidation of lactate to pyruvate [Pubmed|19201793]
Protein family
LutB/YkgF family (single member, according to UniProt)
[wiki|Domains]
2 [wiki|4Fe-4S ferredoxin-type domain]s (aa 304-334, aa 353-382) (according to UniProt)
[wiki|Cofactors]
[4Fe-4S] cluster
Structure
[AF|O07021]
Modification
phosphorylated on Arg-33 [Pubmed|22517742]
Expression and Regulation
Operons
genes
[gene|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[gene|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC]
description
[Pubmed|19201793]
regulation
induction by lactate [Pubmed|19201793]
regulatory mechanism
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|19201793], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR]: repression, in [regulon|protein:E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|lutR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|25031425], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Other regulations
[protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]: translation repression, [Pubmed|22427629,18697947]
[protein|147AFEBEA546FDBEEB08E9A8D9C7BCDC9B83CC90|fbpB]: translation inhibition, acts as RNA chaperone, increases interaction between [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA] and [protein|FEC83B66CEA311EA1650D61A215F82E9DF4E9F03|lutA]-[protein|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]-[protein|5EE69198882DF4E9AB8D9D7267F071EF88C2F16D|lutC] RNAs
Biological materials
Mutant
MGNA-A498 (yvfW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/498 NBRP B. subtilis, Japan]
BKE34040 ([gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCCTTTCCCCCTCTG, downstream forward: _UP4_GAACGCACGAAGGAGGAGCA
BKK34040 ([gene|57B9E37F6232189CD47C1B41FDCF43FCB8016AEB|lutB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34040 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCCTTTCCCCCTCTG, downstream forward: _UP4_GAACGCACGAAGGAGGAGCA
labs
[wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
References
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Time of last update: 2024-11-29 20:45:31
Author of last update: Jstuelk