pnbA
168
para-nitrobenzyl esterase
locus
BSU_34390
Molecular weight
53.82 kDa
pI
4.78
function
lipid degradation
product
para-nitrobenzyl esterase
essential
no
ec
3.1.1.3
synonyms
pnbA, estB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2272 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,530,635 3,532,104
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The protein
Protein family
type-B carboxylesterase/lipase family (single member, according to UniProt)
Structure
[PDB|1C7J]\t[Pubmed|10535917]
[AF|P37967]
Modification
phosphorylation on Ser-189 [Pubmed|17218307]
Expression and Regulation
Operons
genes
[gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]
description
[pubmed|22383849]
regulation
repressed during logarithmic growth ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|18840696]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|18840696], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
genes
[gene|920F91E748EE079FF864011D9052B073567C41E4|slrR]-[gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]
description
[Pubmed|21815947]
regulatory mechanism
[protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: activation, [Pubmed|19767430], in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|19788541], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
additional information
induction by sequestration of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] by [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI], [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|slrA] [PubMed|15661000,19788541] or by SlrR itself [PubMed|20351052]
Biological materials
Mutant
GP951 (''pnbA''::''cat''), available in [wiki|Jörg Stülke]'s lab
GP955 (''[gene|920F91E748EE079FF864011D9052B073567C41E4|slrR]''-''pnbA''::''cat''), available in [wiki|Jörg Stülke]'s lab
BKE34390 ([gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT, downstream forward: _UP4_TAAATATGGGGAAAACAGGA
BKK34390 ([gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTGTTCTCTCCCTTTT, downstream forward: _UP4_TAAATATGGGGAAAACAGGA
References
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Time of last update: 2024-08-20 06:19:09
Author of last update: Jstuelk