purR

purR
168

transcription repressor of the pur operon

locus
BSU_00470
Molecular weight
31.08 kDa
pI
5.77
Protein length
285 aaSequenceBlast
Gene length
858 bpSequenceBlast
function
regulation of purine biosynthesis
product
transcription repressor of the pur operon
essential
no
synonyms
purR, yabI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
54,441  55,298
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The protein
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
[wiki|Domains]
N-terminal winged helix-turn-helix domain [pubmed|34967415]
C-terminal effector binding domain [pubmed|34967415]
Structure
[PDB|1O57] (the apo-protein) [Pubmed|12837783]
[PDB|7RMW] (complex with ppGpp) [pubmed|34967415]
[AF|P37551]
Effectors of protein activity
PRPP acts as inducer, binding results in release of PurR from the operator [Pubmed|10919400]
(p)ppGpp acts as an anti-inducer, prevents binding of the inducer PRPP [pubmed|34967415]
Kinetic information
KD for ppGpp: 4.4 µM [pubmed|34967415]
Expression and Regulation
Operons
genes
[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
description
[Pubmed|22383849]
regulation
negative autoregulation [Pubmed|7638212]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [Pubmed|7638212], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
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[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]→[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]

2024-12-04 20:21:54

Jstuelk

73

620be59868c14a2ea15f75e6fdf1022628ca65bb

B42ADE39EB92F53783BD0BDEF129E37859FEBF47

Biological materials
Mutant
BKE00470 ([gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCCCCAAAAT,  downstream forward: _UP4_TTAAAGAATGGAGAGACAGA
BKK00470 ([gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCCCCAAAAT,  downstream forward: _UP4_TTAAAGAATGGAGAGACAGA
References
Reviews
28031352
Original Publications
10368157,14594850,15629952,11591660,12837783,7638212,12837784,19446032,10919400,34967415

A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C

Page visits: 4639

Time of last update: 2024-12-04 10:53:12

Author of last update: Jstuelk