purQ
168
phosphoribosylformylglycinamidine synthase
locus
BSU_06470
Molecular weight
24.64 kDa
pI
4.63
function
purine biosynthesis
product
phosphoribosylformylglycinamidine synthase
essential
no
ec
6.3.5.3
synonyms
purQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0047 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
702,570 → 703,253
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The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate(according to UniProt)
H2O + L-glutamine --> L-glutamate + NH4+ (according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-1 domain] (aa 3-225) (according to UniProt)
Structure
[PDB|3D54] (from ''Thermotoga maritima'', 51% identity) [Pubmed|18597481]
[AF|P12041]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]-[gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]-[gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]-[gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]-[gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]-[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]-[gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]-[gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]-[gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]-[gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]-[gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]-[gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]
description
[Pubmed|3036807]
regulation
expression activated by glucose (4.4 fold) [Pubmed|12850135]
regulatory mechanism
G-box: RNA switch, ([wiki|riboswitch]) [pubmed|3036807,12787499], in [regulon|other_regulator:G-box|G-box]
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|7638212,2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3036807], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE06470 (Δ[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGGTAACACAATCACCGCAA, downstream forward: _UP4_ATCGTGAAAAATTGGAGGGA
BKK06470 (Δ[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGGTAACACAATCACCGCAA, downstream forward: _UP4_ATCGTGAAAAATTGGAGGGA
References
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Time of last update: 2024-07-14 12:42:58
Author of last update: Melvin.boenninger