lytF
168
gamma-D-glutamate-meso-diaminopimelate muropeptidase, cell separase (major autolysin)
locus
BSU_09370
Molecular weight
51.23 kDa
pI
10.41
function
cell separation
product
gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
essential
no
ec
3.4.-.-
synonyms
lytF, cwlE, yhdD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1388 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,011,792 → 1,013,258
Phenotypes of a mutant
cell separation defect, this is increased by a ''[gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE]'' mutation [Pubmed|23855774]
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The protein
Catalyzed reaction/ biological activity
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]
degradation of gamma-polyglutamic acid [pubmed|29458655]
Protein family
[wiki|Peptidase C40 family] (according to UniProt)
[wiki|Domains]
contains five N-acetylglucosamine-polymer-binding [wiki|LysM domain]s [Pubmed|18430080]
C-terminal D,L-endopeptidase domain ([wiki|NlpC/P60 domain]) [pubmed|29458655,22139507]
5 [wiki|LysM domain]s (aa 27-70, aa 92-135, aa 174-217, aa 240-283, aa 307-350) (according to UniProt)
Structure
[PDB|4XCM] (from Thermus thermophilus, 31% identity) [pubmed|25760608]
[AF|O07532]
Effectors of protein activity
both enzymatic activities are inhibited by interaction with [protein|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] [pubmed|29458655]
[wiki|Localization]
secreted (according to Swiss-Prot)
localizes to cell septa and poles on the vegetative cell surface, this depends on the presence of lipoteichoic acids ([protein|418900B6DBDF3EF300C930B0C75E31B939F3CE00|ltaS]) [Pubmed|25288647,22139507]
for an analysis of motion of [protein|E8B88CBE4F9121DFEEF099D7947CD1E1AE656160|lytF], see [pubmed|38381561]
Expression and Regulation
Operons
genes
[gene|E8B88CBE4F9121DFEEF099D7947CD1E1AE656160|lytF]
description
[Pubmed|10322020]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|920F91E748EE079FF864011D9052B073567C41E4|slrR]: repression, in [regulon|protein:920F91E748EE079FF864011D9052B073567C41E4|slrR regulon]
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|10322020], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
1A791 ( ''lytF''::''spec''), [Pubmed|10206711], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A791&Search=1A791 BGSC]
1A792 ( ''lytF''::''spec''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
BKE09370 (Δ[gene|E8B88CBE4F9121DFEEF099D7947CD1E1AE656160|lytF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAGCTCTCCTTTTTC, downstream forward: _UP4_TAAAAACAGAAACTGTGCGG
BKK09370 (Δ[gene|E8B88CBE4F9121DFEEF099D7947CD1E1AE656160|lytF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAGCTCTCCTTTTTC, downstream forward: _UP4_TAAAAACAGAAACTGTGCGG
References
Reviews
Original Publications
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Time of last update: 2024-11-11 20:19:03
Author of last update: Jstuelk