mutM
168
formamidopyrimidine-DNA glycosidase, part of the error prevention oxidized guanine system (with [protein|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY] and [protein|50A50E33796EEF8046142EE928753C355EF42A1A|rppH])
locus
BSU_29080
Molecular weight
31.06 kDa
pI
8.89
function
DNA repair
product
formamidopyrimidine-DNA glycosidase
essential
no
ec
3.2.2.23
synonyms
mutM, ytaE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0266 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,972,329 → 2,973,159
Phenotypes of a mutant
increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage [Pubmed|24973075]
absence of MutM promotes mutagenesis allowing nutritionally stressed ''B. subtilis'' cells to escape from growth limiting conditions [Pubmed|25825434]
increased sponteous mutation rate [Pubmed|25825434]
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The protein
Catalyzed reaction/ biological activity
binds to abasic and RNA base DNA lesions [pubmed|37250769]
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (according to UniProt)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to UniProt)
Protein family
FPG family (single member, according to UniProt)
Structure
[PDB|2F5S] (complex with oxoG:C, Geobacillus stearothermophilus)
[AF|O34403]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|coaE]
description
[pubmed|22383849]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|23396918], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-A116 (mutM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/116 NBRP B. subtilis, Japan]
BKE29080 (Δ[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCCATCACTTCCTATC, downstream forward: _UP4_TAGCATAATGCTTCATTGTC
BKK29080 (Δ[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29080 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CACATCCATCACTTCCTATC, downstream forward: _UP4_TAGCATAATGCTTCATTGTC
References
Reviews
Original Publications
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Time of last update: 2024-11-29 20:46:04
Author of last update: Jstuelk