ykuN
168
flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation, probably involved in electron transfer to nitric oxide synthase
locus
BSU_14150
Molecular weight
17.65 kDa
pI
3.83
function
electron transfer
product
flavodoxin
essential
no
synonyms
ykuN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0716 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,487,038 1,487,514
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The protein
Protein family
flavodoxin family (with [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP], according to UniProt)
[wiki|Domains]
[wiki|Flavodoxin-like domain] (aa 4-144) (according to UniProt)
[wiki|Cofactors]
FMN [Pubmed|25194416]
Structure
[PDB|5LJL] (flavodoxin from Streptococcus pneumoniae, 42% identity) [pubmed|27649488]
[AF|O34737]
Paralogous protein(s)
[protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]
Expression and Regulation
Operons
genes
[gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]-[gene|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|ykuO]-[gene|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]
description
[Pubmed|12354229]
regulation
expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]) [Pubmed|24214949]
induced by iron starvation (second wave) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [pubmed|29133393,12354229]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|24214949], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]: repression, in [regulon|protein:65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre regulon]
[protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|24214949], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12354229], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [pubmed|29914988], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
additional information
the ''[protein|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]-[protein|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|ykuO]-[protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]'' operon is strongly unregulated in a ''[wiki|kre]'' mutant [Pubmed|26110430]
Biological materials
Mutant
MGNA-B337 (ykuN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1336 NBRP B. subtilis, Japan]
BKE14150 ([gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCCCATTAGT, downstream forward: _UP4_GATTATATGAACAAGGAAAA
BKK14150 ([gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCCCATTAGT, downstream forward: _UP4_GATTATATGAACAAGGAAAA
References
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Time of last update: 2024-12-04 15:18:24
Author of last update: Melvin.boenninger