ansR
168
transcriptional repressor ([wiki|Xre family]) of the [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB] operon
locus
BSU_23590
Molecular weight
13.09 kDa
pI
4.81
function
negative regulation of the [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB] operon
product
transcriptional regulator ([wiki|Xre family])
essential
no
synonyms
ansR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1396 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,456,990 2,457,340
Phenotypes of a mutant
the [gene|search|ansR ]mutation allows glutamate utilization of a ''[gene|56CBEBCFEF5CFB4A0175498338C7AF2F45EAA3E3|rocG] [gene|C36C8C9EEDCAD392D9BEC9728A1E0CBFF2F4E790|gudB]'' mutant [Pubmed|21219666]
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The protein
Protein family
[wiki|Xre family]
[wiki|Domains]
[wiki|HTH cro/C1-type domain] (aa 6-60) (according to UniProt)
Structure
[AF|Q07683]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]
description
[Pubmed|8478318]
regulation
induced in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: negative autoregulation, undefined, in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
Biological materials
Mutant
GP4166 (''ansR''::''cat''), available in [wiki|Jörg Stülke]'s lab
GP1152 (Δ[gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]::tet) available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
BKE23590 ([gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTTCCGCTCCTTTT, downstream forward: _UP4_GAGGAACTGAGTTAATCTTT
BKK23590 ([gene|146947C974D62BCB282BA3F5B924B35695D75886|ansR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTTCCGCTCCTTTT, downstream forward: _UP4_GAGGAACTGAGTTAATCTTT
Expression vectors
expression of native ''ansR'' in ''B. subtilis'': pGP873 (in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-11-20 07:07:00
Author of last update: Jstuelk