spo0A
168
[wiki|phosphorelay] regulator, initiation of [wiki|sporulation], coordinates [wiki|DNA replication] and initiation of [wiki|sporulation] by binding to sites close to the oriC
locus
BSU_24220
Molecular weight
29.54 kDa
pI
5.99
function
initiation of [wiki|sporulation]
product
[wiki|phosphorelay] response regulator
essential
no
synonyms
spo0A, spo0C, spo0G, spoIIL, sof-1
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5801 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,518,023 2,518,826
Phenotypes of a mutant
inactivation of ''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]'' restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]
altered cell death pattern in colonies [Pubmed|23012477]
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The protein
Catalyzed reaction/ biological activity
activation of gene expression at the onset of [wiki|sporulation] ([wiki|Spo0A regulon])
coordination between [wiki|DNA replication] and initiation of [wiki|sporulation] by binding to sites close to the ''oriC'' (this limits re-initiation of replication) [Pubmed|23301687]
required for [wiki|biofilm formation] [Pubmed|11886552]
required for [wiki|sliding] [Pubmed|26152584]
[wiki|Domains]
[wiki|Response regulatory domain] (aa 5-123) (according to UniProt)
Structure
[PDB|1QMP] (with phosphorylated aspartate, ''Geobacillus stearothermophilus''), [PDB|1FC3] (trans-activation domain, ''Geobacillus stearothermophilus''), [PDB|1LQ1] (complex with DNA)
[AF|P06534]
Modification
receives phosphorylation from [protein|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B], dephosphorylation by [protein|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E], direct phosphorylation by [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC] upon potassium leakage [Pubmed|19114652]
phosphorylation is enhanced in a [gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho] mutant [pubmed|28723971]
Effectors of protein activity
the phosphorylation state affects DNA binding activity
interaction with [protein|331993A875907C10C77105FD8DDD86D4412CE405|mecA] inhibits the transcriptional activity of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]P [Pubmed|21435029]
transcription activation by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P is inhibited by interaction with [protein|331993A875907C10C77105FD8DDD86D4412CE405|mecA] (in complex with [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]) [pubmed|29446505]
Expression and Regulation
Operons
genes
[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]
description
[Pubmed|11254139]
regulation
expression increases when drowth rate declines [Pubmed|21552330]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|1391039], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11254139,1569009], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|11254139,1569009], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
additional information
efficient translation depends on [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] [pubmed|36651772]
Biological materials
Mutant
MGNA-C448 (spo0A::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2446 NBRP B. subtilis, Japan]
BKG9 (''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
SS995 (''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]''::''ery'') [Pubmed|14761993]
BKE24220 ([gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTCTTCCTCCCCAAA, downstream forward: _UP4_TAAACATGAGCTTATTAAGT
BKK24220 ([gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTCTTCCTCCCCAAA, downstream forward: _UP4_TAAACATGAGCTTATTAAGT
3NA (spo0A3), available at [http://bgsc.org/getdetail.php?bgscid=1S1 BGSC], [Pubmed|4986704], [Pubmed|25767229], [https://www.ncbi.nlm.nih.gov/nuccore/CP010314 Genome Sequence]
labs
[wiki|Tony Wilkinson], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
[wiki|Imrich Barak], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
References
Reviews
Original Publications
The [wiki|Spo0A regulon]
Page visits: 22491
Time of last update: 2024-12-06 13:05:10
Author of last update: Jstuelk