sigI
168
[wiki|RNA polymerase ][wiki|sigma factor ]SigI
locus
BSU_13450
Molecular weight
29.04 kDa
pI
8.31
function
control of a class of heat shock genes
product
[wiki|RNA polymerase ][wiki|sigma factor ]SigI
essential
no
synonyms
sigI, ykoZ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1191 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,411,892 1,412,647
Phenotypes of a mutant
temperature-sensitive growth on agar plates [Pubmed|11157964]
a [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] mutant is not viable, due to lack of expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] [Pubmed|36265902]
a [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] double mutant is not viable due to its inability to repair gaps in the peptidoglycan mesh [Pubmed|36265902], the growth defect can be suppressed by reduced fatty acid synthesis (mutations in [gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB], [gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC], [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD] or downregulation of the [wiki|FapR regulon] due to specific mutations in [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [pubmed|37017514]
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The protein
Protein family
[wiki|Sigma-70 factor family] (according to UniProt)
Structure
[PDB|6IVU] (from Hungateiclostridium thermocellum, corresponds to aa 141 ... 245, 38% identity) [pubmed|31106374]
[AF|O31654]
[wiki|Localization]
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000194 PRODORIC2 database]
Additional information
overexpression of SigI suppresses ''[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [gene|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|mreBH] [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl]'' mutants [Pubmed|19114499]
Expression and Regulation
Operons
genes
[gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]-[gene|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI]
description
[Pubmed|17185538]
regulation
induced at high temperature ([protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI], [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]) [Pubmed|23199363,17185538]
regulatory mechanism
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|24125693,23199363], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
sigma factors
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [Pubmed|17185538], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|23199363], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A229 (ykoZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/229 NBRP B. subtilis, Japan]
BKE13450 ([gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTCAGTTCCTCCCTATA, downstream forward: _UP4_TATCTTAAAGGGGTGCTGCA
BKK13450 ([gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTCAGTTCCTCCCTATA, downstream forward: _UP4_TATCTTAAAGGGGTGCTGCA
References
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Time of last update: 2024-08-13 10:56:27
Author of last update: Jstuelk