abrB
168
transcriptional regulator of transition state genes
locus
BSU_00370
Molecular weight
10.63 kDa
pI
6.57
function
regulation of gene expression during the transition from growth to stationary phase
product
transcriptional regulator
essential
no
synonyms
abrB, cpsX, tolB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2002 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
44,848 → 45,138
Phenotypes of a mutant
No swarming motility on B medium [Pubmed|19202088]
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The protein
[wiki|Domains]
[wiki|SpoVT-AbrB domain] (aa 7-52) (according to UniProt)
Structure
[PDB|2MJG] (full-length protein) [Pubmed|25308864]
[PDB|1Z0R] (N-terminal DNA recognition domain) [Pubmed|16223496]
[AF|P08874]
Modification
phosphorylated on Ser-86 [Pubmed|20509597] by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC], [protein|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|prkD], and [protein|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT] results in loss of DNA-binding activity [Pubmed|24731262]
Effectors of protein activity
interaction with [protein|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA] results in inactivation of AbrB [Pubmed|18840696]
Paralogous protein(s)
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT], [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]
Expression and Regulation
Operons
genes
[gene|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]
description
[Pubmed|3145384]
regulation
expressed at the onset of stationary phase [Pubmed|3145384]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, -P [Pubmed|3145384], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|2504584], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3145384], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
degradation of the ''[wiki|abrB]'' mRNA is triggered by base-pairing with [wiki|RnaC] [Pubmed|25790031]
Biological materials
Mutant
TT731 (aphA3)
1A935 (''abrB''::''cat''), [Pubmed|11886552], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A935&Search=1A935 BGSC]
GP1548 (''abrB''::''ermC''), available in [wiki|Stülke] lab
BKE00370 (Δ[gene|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTCCCAAGAGATA, downstream forward: _UP4_TAATCATTTCTTGTACAAAA
BKK00370 (Δ[gene|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCTCCCAAGAGATA, downstream forward: _UP4_TAATCATTTCTTGTACAAAA
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Stülke] lab
labs
[wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
[wiki|Mark Strauch], Baltimore, USA [http://lifesciences.umaryland.edu/Pages/faculty_profile.aspx?ID=212 homepage]
References
Reviews
Original Publications
The [wiki|AbrB regulon]
Page visits: 20035
Time of last update: 2024-08-22 07:23:43
Author of last update: Jstuelk