nsrR
168
nitric oxide-responsive regulator
locus
BSU_09380
Molecular weight
16.47 kDa
pI
7.75
function
repression of [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]-dependent genes in the absence of nitric oxide (NO)
product
transcriptional repressor
essential
no
synonyms
nsrR, yhdE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1959 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,013,411 → 1,013,851
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The protein
[wiki|Domains]
[wiki|HTH rrf2-type domain] (aa 2-133) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster, required for DNA-binding [Pubmed|21091510]
Structure
[PDB|3LWF] (from Listeria innocua, 29% identity)
[AF|O07573]
Effectors of protein activity
nitric oxide (NO) acts as molecular inducer and results in NsrR release from its DNA targets [Pubmed|19006327]
[4Fe-4S]-NsrR binds around the -35 element of the ''[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]'' promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO [Pubmed|21091510]
Expression and Regulation
Operons
genes
[gene|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]
description
[Pubmed|10322020]
Biological materials
Mutant
MGNA-A692 (yhdE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/692 NBRP B. subtilis, Japan]
BKE09380 (Δ[gene|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAGACCTCAGAATA, downstream forward: _UP4_TAGATTAAGATTCCTTCTTT
BKK09380 (Δ[gene|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAGACCTCAGAATA, downstream forward: _UP4_TAGATTAAGATTCCTTCTTT
References
Reviews
Other original publications
The [wiki|NsrR regulon]
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Time of last update: 2024-11-11 11:06:33
Author of last update: Melvin.boenninger