McsB

• Description: protein arginine kinase, adaptor protein, modulator of CtsR-dependent repression
  
  

Gene name	mcsB
Synonyms	yacI
Essential	no
Product	protein arginine kinase
Function	control of CtsR activity
Gene expression levels in SubtiExpress: mcsB
Interactions involving this protein in SubtInteract: McsB
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	40 kDa, 5.068
Gene length, protein length	1089 bp, 363 aa
Immediate neighbours	mcsA, clpC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigF regulon

The gene

Basic information

• Locus tag: BSU00850

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: targets non-functional CtsR for degradation by ClpP/ClpC PubMed

• Protein family: ATP:guanido phosphotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • autophosphorylation on specific arginine residues PubMed, dephosphorylation by YwlE PubMed
  • autophosphorylation stimulates McsB activity as adaptor protein for ClpC PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • McsA-McsB-CtsR, McsB-CtsR PubMed, McsA-McsB
  • McsB-YwlE PubMed
  • McsB-ClpP/ClpC (rapid degradation of phosphorylated McsB) PubMed

• Localization:

Database entries

• Structure:

• UniProt: P37570

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Expression browser: mcsB PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2, SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed
  • induced by stress (SigB) PubMed
  • induced by heat (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • mcsB::aphA3 availbale from the Gerth lab
  • mcsBC167S::spec available from the Gerth lab
  • GP1457 (mcsB::aphA3), available in Stülke lab
  • BP69 (spc), available in Fabian Commichau's lab
• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Gerth lab

Labs working on this gene/protein

• Ulf Gerth, Greifswald, Germany

• Fabian Commichau Göttingen, Germany

References

Reviews

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260] [WorldCat.org] [DOI] (I p)